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Levels of carnitine and acylcarnitines in reconstituted red blood cell samples washed with different concentrations of saline solutions

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dc.creator Osorio, José Henry
dc.creator Pourfarzam, Morteza
dc.date.accessioned 2011-11-11T15:10:14Z
dc.date.available 2011-11-11T15:10:14Z
dc.date.issued 2011-11-11
dc.identifier.uri http://hdl.handle.net/10893/3170
dc.description.abstract Objective: To evaluate the percentage of carnitine and acylcarnitines remaining in red blood cells after washing them with different concentrations of saline solution. Materials and methods: Human blood samples were centrifuged and the blood cells were washed with different saline solutions. The final pellet was resuspended in PBS for card preparation and tandem mass spectrometry analysis. Results: It was found that carnitine, as well as short-chain, medium-chain, and long-chain acylcarnitines remain in red blood cells at average percentages of 19.3; 34; 34; and 32%, respectively. Significant differences were found for carnitine and acylcarnitine levels in blood washed with an isotonic solution compared to their levels using several hypotonic solutions (p<0.05). Conclusion: Because carnitine and acylcarnitines remained associated with the blood cells, we recommend using whole blood to measure these metabolites. Objetivo: Evaluar el porcentaje de carnitina y acilcarnitinas que permanece en los glóbulos rojos, luego de lavarlos con solución salina a diferentes concentraciones. Materiales y métodos: Muestras de sangre humana fueron centrifugadas y los glóbulos rojos fueron lavados con solución salina a diferentes concentraciones. El pellet obtenido, fue resuspendido en PBS para preparación de tarjetas y análisis por espectrometría de masa en tandem. Resultados: La carnitina, así como las acilcarnitinas de cadena corta, cadena media y cadena larga, permanecen en los glóbulos rojos a porcentajes promedio de 19,3; 34; 34; and 32%, respectivamente. Se encontró diferencia significativa al comparar los niveles de carnitina y acilcarnitinas de sangre lavada con una solución salina isotónica vs. varias hipotónicas (p<0.05). Conclusión: debido a que la carnitina y las acilcarnitinas permanecen en los glóbulos rojos, se recomienda el uso de sangre entera para medir los niveles de estos metabolitos. spa
dc.language.iso en_US spa
dc.subject Carnitine spa
dc.subject Acylcarnitines spa
dc.subject Acyl-CoA spa
dc.subject Tandem mass spectrometry spa
dc.subject Red blood cells spa
dc.subject Metabolism spa
dc.subject Carnitina spa
dc.subject Acylcarnitinas spa
dc.subject Acyl-CoA spa
dc.subject Espectrometría de masa en tándem spa
dc.subject Glóbulos rojos spa
dc.subject Metabolismo spa
dc.title Levels of carnitine and acylcarnitines in reconstituted red blood cell samples washed with different concentrations of saline solutions spa
dc.title.alternative Niveles de carnitina y acilcarnitinas en muestras de glóbulos rojos reconstituidos y lavados con solución salina a diferentes concentraciones spa
dc.type Article spa
dc.rights.accessrights openAccess spa


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