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dc.contributor.authorSoto Franco, Jorge Enriquespa
dc.contributor.authorContreras Rengifo, Adolfospa
dc.contributor.authorParra Patiño, Beatrizspa
dc.date.accessioned2011-10-18T14:16:56Z
dc.date.available2011-10-18T14:16:56Z
dc.date.issued2011-10-18
dc.identifier.urihttp://hdl.handle.net/10893/2538
dc.description.abstractThe main goal is to establish a cellular line of fibroblast which come from human gingival tissue, identifying its morphologic featores. Samples were taken from gum of healthy patients without medical antecedents. Three females and three male were selected. Ages were between 16 and 30 years old. A sample of 0,5 cm² of gum was obtained to take it to a cellular growth environment. Cultures were incobated at 37C, CO² atmosphere at 5% for ten (10) days. Samples were observed daily with inverted light Microscope Olimpus II. At 6th day growth of fusiform cells was observed; their morphology was in coincidence with that of the fibroblasts, At 18th day a monolayer of 100% purity was observed. The cellular line was preserved and stored by freezing in liquid nitrogen.spa
dc.language.isoesspa
dc.subjectLínea Celularspa
dc.subjectFibroblastosspa
dc.subjectCariotipificaciónspa
dc.subjectCitoesqueletospa
dc.subjectMicrotúbulosspa
dc.subjectEnfermedades Periodontalesspa
dc.subjectEncíaspa
dc.subjectDimetilsulfóxidospa
dc.titleCultivo y Caracterización In Vitro de una Línea Celular de Fibroblastos Gingivales Humanosspa
dc.typeArtículo de revistaspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa


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