Examinando por Materia "Virological surveillance"

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    Vertical transmission of dengue virus in Aedes spp. (Diptera: Culicidae) in Medellín, Colombia.
    (Universidad del Valle, 2020-07-09) Rúa Uribe, Guillermo L.; Giraldo Jaramillo, Tatiana M.; Triana Chávez, Omar; Rojo, Raúl; Henao, Enrique; Pérez Pérez, Juliana; Sociedad Colombiana de Entomología - Socolen; Universidad del Valle; Montoya Lerma, James
    Dengue is the world’s most important arbovirus. It is transmitted by the bite of females Aedes spp. mosquitoes, which normally become infected by biting a viraemic host. There are, however, numerous studies that have identified vertical transmission as a rare yet important mechanism of vector infection. This study was conducted in order to quantify the occurrence of vertical transmission of dengue virus in Aedes mosquitoes collected by the Secretary of Health in Medellin, Colombia in 2016 (epidemic year) and 2017 (endemic year). Specimens were identified to species and tested for dengue in pools by RT-PCR. A total of 6,708 mosquitoes were collected; 96 % were Ae. aegypti, and 4 % were Ae. Albopictus. 294 of 2,211 pools tested were positive for dengue virus, and 50 of the positive pools were exclusively male mosquitoes. For both epidemic and the endemic years, vertical transmission was detected, though it was more frequent in 2016. Vertical transmission was observed in mosquitoes collected from all sectors of the city, without a distinct spatial pattern. Our results suggest that vertical transmission is a more frequent event with greater epidemiological relevance than previously estimated. Additional studies are required to verify the infectivity of the virus detected in male mosquitoes and to specify the role of vertical transmission in the incidence of dengue virus.