Levels of carnitine and acylcarnitines in reconstituted red blood cell samples washed with different concentrations of saline solutions
| dc.contributor.author | Osorio, José Henry | |
| dc.contributor.author | Pourfarzam, Morteza | |
| dc.date.accessioned | 2011-11-11T15:10:14Z | |
| dc.date.available | 2011-11-11T15:10:14Z | |
| dc.date.issued | 2011-11-11 | |
| dc.description.abstract | Objective: To evaluate the percentage of carnitine and acylcarnitines remaining in red blood cells after washing them with different concentrations of saline solution. Materials and methods: Human blood samples were centrifuged and the blood cells were washed with different saline solutions. The final pellet was resuspended in PBS for card preparation and tandem mass spectrometry analysis. Results: It was found that carnitine, as well as short-chain, medium-chain, and long-chain acylcarnitines remain in red blood cells at average percentages of 19.3; 34; 34; and 32%, respectively. Significant differences were found for carnitine and acylcarnitine levels in blood washed with an isotonic solution compared to their levels using several hypotonic solutions (p<0.05). Conclusion: Because carnitine and acylcarnitines remained associated with the blood cells, we recommend using whole blood to measure these metabolites. Objetivo: Evaluar el porcentaje de carnitina y acilcarnitinas que permanece en los glóbulos rojos, luego de lavarlos con solución salina a diferentes concentraciones. Materiales y métodos: Muestras de sangre humana fueron centrifugadas y los glóbulos rojos fueron lavados con solución salina a diferentes concentraciones. El pellet obtenido, fue resuspendido en PBS para preparación de tarjetas y análisis por espectrometría de masa en tandem. Resultados: La carnitina, así como las acilcarnitinas de cadena corta, cadena media y cadena larga, permanecen en los glóbulos rojos a porcentajes promedio de 19,3; 34; 34; and 32%, respectivamente. Se encontró diferencia significativa al comparar los niveles de carnitina y acilcarnitinas de sangre lavada con una solución salina isotónica vs. varias hipotónicas (p<0.05). Conclusión: debido a que la carnitina y las acilcarnitinas permanecen en los glóbulos rojos, se recomienda el uso de sangre entera para medir los niveles de estos metabolitos. | spa |
| dc.identifier.uri | https://hdl.handle.net/10893/3170 | |
| dc.language.iso | en_US | spa |
| dc.rights.accessrights | info:eu-repo/semantics/openAccess | spa |
| dc.subject | Carnitine | spa |
| dc.subject | Acylcarnitines | spa |
| dc.subject | Acyl-CoA | spa |
| dc.subject | Tandem mass spectrometry | spa |
| dc.subject | Red blood cells | spa |
| dc.subject | Metabolism | spa |
| dc.subject | Carnitina | spa |
| dc.subject | Acylcarnitinas | spa |
| dc.subject | Acyl-CoA | spa |
| dc.subject | Espectrometría de masa en tándem | spa |
| dc.subject | Glóbulos rojos | spa |
| dc.subject | Metabolismo | spa |
| dc.title | Levels of carnitine and acylcarnitines in reconstituted red blood cell samples washed with different concentrations of saline solutions | spa |
| dc.title.alternative | Niveles de carnitina y acilcarnitinas en muestras de glóbulos rojos reconstituidos y lavados con solución salina a diferentes concentraciones. | spa |
| dc.type | Artículo de revista | spa |
| dspace.entity.type | Publication |