Rapid Determination of Clavulanic Acid in Submerged Cultures of Streptomyces clavuligerus as an Alternative Method for Bioprocess Monitoring and Control

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dc.contributor.advisorGómez Rios, David Andrés
dc.contributor.advisorMosquera Restrepo, Jaime
dc.contributor.authorJiménez Bravo, Leidy Alejandra
dc.contributor.authorTrochez Cifuentes, Julieth Alexandra
dc.date.accessioned2026-09-15T15:22:08Z
dc.date.available2026-09-15T15:22:08Z
dc.date.issued2026
dc.description.abstractClavulanic acid (CA) is a β-lactamase inhibitor widely used in combination with β-lactam antibiotics to overcome bacterial resistance, and it is produced by Streptomyces clavuligerus through submerged fermentation. To ensure its industrial-scale production, it is essential to develop analytical methods that are rapid, reliable, and suitable for process monitoring. In this work, a UV–VIS spectrophotometric method was developed and validated for the quantification of CA in simulated fermentation matrices, employing ethyl acetate as the solvent for liquid–liquid extraction. The method demonstrated linearity in the range of 100–600 ppm (R² > 0.98), with coefficients of variation below 3% and recovery rates ranging from 90% to 110%. Clavulanic acid remained stable for up to 72 h under refrigeration, and complete transfer to the organic phase was achieved within 40 min. No significant interferences were detected, and the method showed adequate sensitivity (LOD: 60 ppm; LOQ: 100 ppm). These results confirm that the proposed method is a fast, cost-effective, and reproducible alternative to chromatographic techniques such as HPLC, representing a practical tool for routine bioprocess monitoring and quality control in the industrial production of clavulanic acid.eng
dc.description.degreelevelPregradospa
dc.description.degreenameINGENIERO (A) QUÍMICO (A)spa
dc.format.extent1 Recurso en linea (19 páginas)
dc.format.mimetypeapplication/pdf
dc.identifier.notificationemailjimenez.leidy@correounivalle.edu.co
dc.identifier.notificationemailjulieth.trochez@correounivalle.edu.co
dc.identifier.notificationnameLeidy Alejandra Jiménez Bravo, cedula 1107101285
dc.identifier.notificationnameJulieth Alexandra Trochez Cifuentes, cedula 1108511814
dc.identifier.urihttps://hdl.handle.net/10893/41671
dc.language.isoeng
dc.publisherUniversidad del Vallespa
dc.publisher.branchSede Cali
dc.publisher.facultyFACULTAD DE INGENIERÍAspa
dc.publisher.placeColombiaspa
dc.publisher.programINGENIERÍA QUÍMICAspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.coarhttp://purl.org/coar/access_right/c_abf2spa
dc.rights.licenseAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)spa
dc.subject.ddc540 - Química y ciencias afines
dc.subject.lembEspectrofotometría
dc.subject.lembAcetato de etilo
dc.subject.lembExtracción (Química)
dc.subject.lembAcido clavulánico
dc.titleRapid Determination of Clavulanic Acid in Submerged Cultures of Streptomyces clavuligerus as an Alternative Method for Bioprocess Monitoring and Controleng
dc.typeTrabajo de grado - Pregradospa
dc.type.coarhttp://purl.org/coar/resource_type/c_7a1f
dc.type.coarversionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
dc.type.contentText
dc.type.driverinfo:eu-repo/semantics/bachelorThesis
dc.type.redcolhttp://purl.org/redcol/resource_type/TP
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dspace.entity.typePublication
person.identifier.orcid0000-0002-8404-2472
relation.isDirectorOfPublication53c732d1-c67b-4314-a4bd-7ff6fe7b1338
relation.isDirectorOfPublication.latestForDiscovery53c732d1-c67b-4314-a4bd-7ff6fe7b1338
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