Leishmania (Viannia) panamensis expresses a nuclease with molecular and biochemical features similar to the Endonuclease G of higher eukaryotes.
| dc.contributor.author | Toro Londoño, Miguel A. | |
| dc.contributor.author | Patiño, Edwin Bairon | |
| dc.contributor.author | Robledo, Sara María | |
| dc.contributor.author | Jiménez Ruiz, Antonio | |
| dc.contributor.author | Alzate, Juan Fernando | |
| dc.date.accessioned | 2011-11-04T16:00:12Z | |
| dc.date.available | 2011-11-04T16:00:12Z | |
| dc.date.issued | 2011-11-04 | |
| dc.description.abstract | Objective: To characterize the molecular and biochemical features of the Endonuclease G of Leishmania (Viannia) panamensis. Methods: The gene of the putative L. (V.) panamensis Endonuclease G was amplified, cloned, and sequenced. The recombinant protein was produced in a heterologous expression system and biochemical assays were run to determine its ion, temperature, and pH preferences. Results: The L. (V.) panamensis rENDOG has biochemical features similar to those found in other trypanosomatids and higher eukaryotes. In addition, phylogenetic analysis revealed a possible evolutionary relationship with metazoan ENDOG. Conclusions: L. (V.) panamensis has a gene that codifies an ENDOG homologous to those of higher organisms. This enzyme can be produced in Escherichia coli and is able to degrade covalently closed circular double-stranded DNA. It has a magnesium preference, can be inhibited by potassium, and is able to function within a wide temperature and pH range. Objetivo: Caracterizar molecular y bioquímicamente la Endonucleasa G (EndoG) de Leishmania (Viannia) panamensis. Métodos: El gen de la putativa Endonucleasa G de L. (V.) panamensis fue amplificado, clonado y secuenciado. La proteína recombinante se produjo en un sistema de expresión heterólogo y la proteína activa se sometió a pruebas bioquímicas para determinar la preferencia de iones, temperatura y pH. Resultados: La rEndoG de L. (V.) panamensis muestra características bioquímicas similares a aquellas descritas en otros trypanosomatidos y en eucariotas superiores. Además, los análisis filogenéticos muestran una posible relación evolutiva con la Endonucleasa G de metazoos. Conclusiones: Leishmania (V.) panamensis posee un gen que codifica para una endonucleasa homóloga a la EndoG de otros organismos superiores, que se puede producir de forma recombinante en Escherichia coli y que es capaz de degradar ADN circular cerrado de doble cadena. Tiene una preferencia por los iones magnesio y manganeso para usarlos como cofactor y es inhibida por el potasio. Además, funciona en un amplio rango de pH y temperatura. | spa |
| dc.identifier.uri | https://hdl.handle.net/10893/3137 | |
| dc.language.iso | en_US | spa |
| dc.rights.accessrights | info:eu-repo/semantics/openAccess | spa |
| dc.subject | Leishmania panamensis | spa |
| dc.subject | Endonuclease G | spa |
| dc.subject | Molecular characterization | spa |
| dc.subject | Recombinant proteins | spa |
| dc.subject | Refolding | spa |
| dc.subject | Phylogeny | spa |
| dc.subject | Leishmania panamensis | spa |
| dc.subject | Endonucleasa G | spa |
| dc.subject | Caracterización molecular | spa |
| dc.subject | Proteínas recombinantes | spa |
| dc.subject | Renaturalización | spa |
| dc.subject | Filogenia | spa |
| dc.title | Leishmania (Viannia) panamensis expresses a nuclease with molecular and biochemical features similar to the Endonuclease G of higher eukaryotes. | spa |
| dc.title.alternative | Leishmania (Viannia) panamensis expresa una nucleasa molecular y bioquímicamente similar a la Endonucleasa G de eucariotas superiores. | spa |
| dc.type | Artículo de revista | spa |
| dspace.entity.type | Publication |