Hypothenemus hampei (Coleoptera: Curculionidae) as in vitro inducer of laminarinase and chitinase of Beauveria bassiana (Hypocreales: Clavicipitaceae).

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dc.contributor.authorAcuña Payano, Rosalyn
dc.contributor.authorQuiroz Farfán, Diego
dc.contributor.authorLaynes Zela, Pedro
dc.contributor.authorNolasco Cárdenas, Oscar
dc.contributor.authorGutiérrez Román, Ana
dc.contributor.corporatenameSociedad Colombiana de Entomología - Socolenspa
dc.contributor.corporatenameUniversidad del Vallespa
dc.date.accessioned2021-06-29T14:22:33Z
dc.date.available2021-06-29T14:22:33Z
dc.date.issued2017-06-30
dc.description.abstractIn Peru, Hypothenemus hampei or the coffee berry borer, (CBB) is the most important pest, causing specific damage to coffee crops, because it causes fruit to fall during feeding. On the other hand, Beauveria bassiana is an entomopathogenic fungus widely used in biological control. This study determine the induction of chitinase and laminarinase of B. bassiana in presence of H. hampei powder and how it relates to pathogenicity in order to promote the development of biological control agents and replace the use of pesticides. Three different liquid media cultures were prepared to induce the enzymatic production and growth of B. bassiana; and biomass, total proteins, conidia, enzymatic activities and enzymatic specific activities were evaluated. Data were analyzed using SPSS 19.0. Significant differences (P < 0.05) for biomass (F = 5.30; df = 35; P < 0.05), conidia (F = 190.87; df = 35; P < 0.05), total proteins (F = 91.04; df = 35; p < 0.05), laminarinase activity (F = 27.61; df = 35; P < 0.05), specific laminarinase activity (F = 25.31; df = 35; P < 0.05), chitinase activity (F = 32.66; df = 35; P < 0.05) and specific chitinase activity (F = 26.91; df = 35; P < 0.05) were determined between each treatment evaluated. Levels of chitinase and laminarinase determined that induction of these enzymes is possible in liquid media cultures supplemented with powdered H. hampei extract. Results require further evaluation for application in vivo but are fundamental and constitute a contribution to knowledge about the pathogenic mechanism of B. bassiana.spa
dc.format.extent1 recurso en línea (7 páginas)spa
dc.format.mimetypeapplication/pdfspa
dc.identifier.urihttps://hdl.handle.net/10893/20582
dc.language.isospaspa
dc.publisherUniversidad del Vallespa
dc.publisher.placeColombiaspa
dc.relation.citationendpage13spa
dc.relation.citationissue1spa
dc.relation.citationstartpage7spa
dc.relation.citationvolume43spa
dc.relation.ispartofjournalRevista Colombiana de Entomologíaspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.subject.ddcCoffee berry borer
dc.subject.ddcConidia
dc.subject.ddcBiomass
dc.subject.ddcEntomopathogen
dc.subject.ddcEnzyme activity
dc.subject.ddcBroca del café
dc.subject.ddcConidios
dc.subject.ddcBiomasa
dc.subject.ddcEntomopatógeno
dc.subject.ddcActividad enzimática
dc.titleHypothenemus hampei (Coleoptera: Curculionidae) as in vitro inducer of laminarinase and chitinase of Beauveria bassiana (Hypocreales: Clavicipitaceae).spa
dc.typeArtículo de revistaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.redcolhttp://purl.org/redcol/resource_type/ARTspa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dspace.entity.typePublication
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
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