The presence of Micrathyria aequalis (Hagen, 1861), Miathyria marcella (Selys, 1857), Lestes forfícula (Rambur 1842), and Acanthagrion inexpectum (Leonard, 1977) is reported for the first time for the Department of Huila, bringing the total number of Odonata species in this part of Colombia to 29.